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- Title
Anandamide extends platelets survival through CB<sub>1</sub>-dependent Akt signaling.
- Authors
Catani, Maria Valeria; Gasperi, Valeria; Evangelista, Daniela; Agrò, Alessandro Finazzi; Avigliano, Luciana; Maccarrone, Mauro
- Abstract
Platelets are stored at 22°C, since incubation at 37°C results in loss of viability. Nonetheless, in our body (37°C), platelets survive for 8–10 days. This discrepancy has been explained in terms of deprivation of viability factors or accumulation of apoptotic factors during storage. We report that the endocannabinoid anandamide (AEA) may be one of the agents allowing platelet survival. In fact, at 37°C, human platelets enhance the expression of pro-apoptotic proteins (caspases, Bax, Bak) and decrease the expression of Bcl-xL, thus changing the Bcl-xL/Bak ratio, a key platelet biological clock. AEA or its non-hydrolyzable analogue, methanandamide, extend platelet life span, without reversing the changes in Bcl-xL/Bak ratio induced by heat stress. Instead, AEA binding to type-1 cannabinoid receptor activates Akt, which regulates, through phosphorylation of Bad, the interactions among different Bcl-2 family members. These findings could have implications for platelet collection and, potentially, for their clinical use.
- Subjects
CELL death; CELLULAR signal transduction; THROMBOCYTOPENIA; BLOOD platelets; BLOOD cells
- Publication
Cellular & Molecular Life Sciences, 2010, Vol 67, Issue 4, p601
- ISSN
1420-682X
- Publication type
Article
- DOI
10.1007/s00018-009-0198-9