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- Title
The Expression, Clinical Significance of Wip1 in Thyroid Carcinoma and the Biological Effect in Its Cell Line by siRNA Targeting Wip1.
- Authors
W. J. Zhang; G. G. Sun; L. C. Zheng; X. M. Zhang
- Abstract
Objective: This study aimed to analyze the expression, clinical significance of proto-oncogene in thyroid carcinoma and the biological effect in its cell line by siRNA targeting wild-type p53-induced phosphatase 1 (Wip1). Methods: Immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) were respectively used to analyze Wip1 protein expression in 73 cases of thyroid cancer and normal tissues to study the relationship between Wip1 expression and clinical factors. Wip1 siRNA was transiently transfected into papillary thyroid carcinoma cell by liposome-mediated method and was detected by RT-PCR and western blot. MTT assay, cell apoptosis, cell cycle were also conducted as to the influence of the down-regulated expression of Wip1 that might be found on K1 cells biological effect. Results: The positive rates of Wip1 protein was 80.8% in thyroid carcinoma tissues but 9.6% expressed in normal tissues (P < 0.05). The relative content of Wip1 mRNA were (0.665 ± 0.046), (0.225 ± 0.039) in thyroid carcinoma tissues and normal tissues, respectively, with significant differences between the two types (P < 0.05). There was no significant differences between Wip1 expression and sex, age, tumor size (P > 0.05). However, there were significant differences between Wip1 expression and lymph node metastasis, clinical stages and tumor differentiation (P < 0.05). Individuals with high and low levels of Wip1 expression showed statistically significant differences in the ten-year overall survival rate (P < 0.05). RT-PCR and Western blot showed that K1 cell transfected Wip1 siRNA had a lower relative expressive content than normal cell (P < 0.05). MTT assay, cell apoptosis, cell cycles demonstrated that K1 cell transfected Wip1 siRNA had a lower survival fraction, higher cell apoptosis, more percentage of the G0/G1 phases, and lower cells in the G2/M and S phases (P < 0.05). Conclusion: Wip1 protein and mRNA were increased in thyroid carcinoma, specifically in lymph node metastasis, clinical stages and tumor differentiation. Wip1 may be involved in the biological processes of thyroid cancer cell proliferation, apoptosis, and cell cycle.
- Subjects
CHINA; APOPTOSIS; BIOLOGICAL assay; CANCER; CELL culture; CELL cycle; CHI-squared test; FLOW cytometry; IMMUNOHISTOCHEMISTRY; RESEARCH methodology; PHOSPHATASES; POLYMERASE chain reaction; RNA; T-test (Statistics); THYROID gland tumors; TISSUE culture; WESTERN immunoblotting; DATA analysis software; DESCRIPTIVE statistics; KAPLAN-Meier estimator
- Publication
International Medical Journal, 2014, Vol 21, Issue 1, p60
- ISSN
1341-2051
- Publication type
Article