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- Title
Whole-Genome Sequencing to Predict Antimicrobial Susceptibility Profiles in Neisseria gonorrhoeae.
- Authors
Bristow, Claire C; Mortimer, Tatum D; Morris, Sheldon; Grad, Yonatan H; Soge, Olusegun O; Wakatake, Erika; Pascual, Rushlenne; Murphy, Sara McCurdy; Fryling, Kyra E; Adamson, Paul C; Dillon, Jo-Anne; Parmar, Nidhi R; Le, Hai Ha Long; Le, Hung Van; Ureña, Reyna Margarita Ovalles; Mitchev, Nireshni; Mlisana, Koleka; Wi, Teodora; Dickson, Samuel P; Klausner, Jeffrey D
- Abstract
Background Neisseria gonorrhoeae is a major public health problem due to increasing incidence and antimicrobial resistance. Genetic markers of reduced susceptibility have been identified; the extent to which those are representative of global antimicrobial resistance is unknown. We evaluated the performance of whole-genome sequencing (WGS) used to predict susceptibility to ciprofloxacin and other antimicrobials using a global collection of N. gonorrhoeae isolates. Methods Susceptibility testing of common antimicrobials and the recently developed zolifodacin was performed using agar dilution to determine minimum inhibitory concentrations (MICs). We identified resistance alleles at loci known to contribute to antimicrobial resistance in N. gonorrhoeae from WGS data. We tested the ability of each locus to predict antimicrobial susceptibility. Results A total of 481 N. gonorrhoeae isolates, collected between 2004 and 2019 and making up 457 unique genomes, were sourced from 5 countries. All isolates with demonstrated susceptibility to ciprofloxacin (MIC ≤0.06 μg/mL) had a wild-type gyrA codon 91. Multilocus approaches were needed to predict susceptibility to other antimicrobials. All isolates were susceptible to zoliflodacin, defined by an MIC ≤0.25 μg/mL. Conclusions Single marker prediction can be used to inform ciprofloxacin treatment of N. gonorrhoeae infection. A combination of molecular markers may be needed to determine susceptibility for other antimicrobials.
- Subjects
NEISSERIA gonorrhoeae; NUCLEOTIDE sequencing; DRUG resistance in microorganisms; DISC diffusion tests (Microbiology); GENETIC markers
- Publication
Journal of Infectious Diseases, 2023, Vol 227, Issue 7, p917
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1093/infdis/jiad027