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- Title
Interaction Between the Antibiotic Tetracycline and the Elongation Factor 1α from the Archaeon Sulfolobus solfataricus.
- Authors
Lamberti, Anna; Martucci, Nicola M.; Ruggiero, Immacolata; Arcari, Paolo; Masullo, Mariorosario
- Abstract
The interaction between tetracycline and the archaeal elongation factor 1a from Sulfolobus solfataricus was investigated. The effects produced by this eubacterial antibiotic indicated that this interaction involved the G-domain of the elongationfactor 1a from S. solfataricus, although also the Mdomain was required. In fact, in the presence of the antibiotic, an increase in the fluorescence quantum yield of the aromatic region was observed for elongation factor 1afrom S. solfataricus and its truncated form lacking the C-terminal domain, but not for that lacking also the M-domain. The increase in quantum yield was restored when the G-domain of elongation factor 1a from S. solfataricus was fused to the M and the C-domains of the eubacterial analogue elongation factorTu. Tetracycline inhibits protein synthesis catalysed by elongation factor 1a from S. solfataricus; this is accompanied by an increase in the GDP/GTPexchange rate and a slight inhibition of the intrinsic GTPase, suggesting that a main effect of the antibiotic was exerted on theGTP-bound form of the enzyme. Furthermore, the mixed inhibition observed for GTPase confirmed that the interaction, besides the G-domain, involved also other region(s) of elongation factor 1a from S. solfataricus. These results can be useful for studying potential side effects arising from the interaction between tetracycline and eukaryotic elongation factors.
- Subjects
TETRACYCLINE; G proteins; GUANOSINE triphosphatase; TRANSFER RNA; FLUORESCENCE spectroscopy; CHEMICAL biology
- Publication
Chemical Biology & Drug Design, 2011, Vol 78, Issue 2, p260
- ISSN
1747-0277
- Publication type
Article
- DOI
10.1111/j.1747-0285.2011.01142.x