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- Title
Over-expression of miR-124 impairs proliferation, invasion and epithelial-mesenchymal transition in pituitary prolactinoma by targeting PHD finger protein 19.
- Authors
Zongxi Li; Lingxuan Ren; Shoujie Wang
- Abstract
Purpose: To determine the effect of miR-124 in pituitary prolactinoma. Methods: The viability and proliferation of prolactinoma cells were investigated using Cell Counting Kit-8 and 5-ethynyl-2'-deoxyuridine staining assays. Cell migration and invasion were investigated using the transwell assay. The epithelial-mesenchymal transition was investigated using western blotting. The target gene of miR-124 was verified by the luciferase activity assay. Results: The viability and proliferation of prolactinoma cells were repressed by miR-124 overexpression (p < 0.01). Forced miR-124 expression suppressed prolactinoma cell migration and invasion (p < 0.01). E-cadherin expression was enhanced, while N-cadherin and vimentin were reduced, by miR-124 over-expression (p < 0.01). PHF19 (plant homeodomain-like finger protein 19) contains an miR-124 binding site, and PHF19 over-expression enhanced cell proliferation, promoted cell migration and invasion, reduced E-cadherin expression and enhanced N-cadherin and vimentin expression in prolactinoma cells. Additionally, miR-124 mimic-induced suppression of prolactinoma cell growth and metastasis was attenuated by forced PHF19 expression. Conclusion: MiR-124 retards prolactinoma cell growth and metastasis by reducing PHF19, providing a promising therapeutic target for prolactinoma.
- Subjects
EPITHELIAL-mesenchymal transition; PROLACTINOMA; CELL migration; CELL growth; PITUITARY adenylate cyclase activating polypeptide
- Publication
Tropical Journal of Pharmaceutical Research, 2021, Vol 20, Issue 4, p721
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v20i4.9