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- Title
A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a Bio-nanocapsule via an endocytic uptake pathway.
- Authors
Yuya Nishimura; Koichi Takeda; Ryosuke Ezawa; Jun Ishii; Chiaki Ogino; Akihiko Kondo
- Abstract
Background: An affibody-displaying bio-nanocapsule (ZHER2-BNC) with a hepatocyte specificity derived from hepatitis B virus (HBV) was converted into an affibody, ZHER2, that recognizes HER2 receptors. This affibody was previously reported to be the result of the endocytosis-dependent specific uptake of proteins and siRNA into target cancer cells. To assist the endosomal escape of inclusions, a helper lipid with pH-sensitive fusogenic ability (1,2-dioleoyl-sn-glycero-3-phos phoethanolamine; DOPE) was conjugated with a ZHER2-BNC. Findings: In this study, we displayed a pH-sensitive fusogenic GALA peptide on the surface of a particle in order to confer the ability of endosomal escape to a ZHER2-BNC. A GALA-displaying ZHER2-BNC purified from yeast uneventfully formed a particle structure. Furthermore, endosomal escape of the particle was facilitated after endocytic uptake and release of the inclusions to the cytoplasm without the cell toxicity. Conclusion: The genetic fusion of a GALA peptide to the virus-like particle confers the ability of endosomal escape.
- Publication
Journal of Nanobiotechnology, 2014, Vol 12, Issue 1, p11
- ISSN
1477-3155
- Publication type
Article
- DOI
10.1186/1477-3155-12-11