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- Title
miR-182-5p affects human bladder cancer cell proliferation, migration and invasion through regulating Cofilin 1.
- Authors
Wang, Fei; Wu, Dinglan; Xu, Zhanping; Chen, Jianxiang; Zhang, Jiye; Li, Xiaojuan; Chen, Shiliang; He, Fengrong; Xu, Jianbing; Su, Liangju; Luo, Defan; Zhang, Shufang; Wang, Weifu
- Abstract
Background: Human bladder cancer is one of the common malignant tumors, and it mainly occurs in men. miR-182-5p, a member of miR-183 family, acts as tumor suppressor or oncogene in various kinds of tumors. In this study, we first investigate that the absence of miR-182-5p in human bladder cancer promotes tumor growth by regulating the expression of Cofilin 1, an actin modulating-protein. Methods: Human bladder tumor tissue specimens were collected to detect the expression of miR-182-5p and Cofilin 1 by qRT-PCR. Luciferase activity assay was performed to demonstrate the regulation of Cofilin 1 mRNA 3′UTR by miR-182-5p. Then, cell experiments were performed to analysis the effect of miR-182-5p/Cofilin 1 pathway on tumor cell proliferation, migration, invasion and colony forming efficiency. Finally, xenograft tumor models were established to evaluate the role of miR-182-5p in tumorigenesis abilities in vivo. Results: qRT-PCR and Western blotting analysis showed that Cofilin 1 expression was up-regulated in both bladder cancer tissues and cell lines compared with normal. Luciferase activity assay showed that miR-182-5p specifically targets Cofilin 1 mRNA 3′UTR and represses the expression of Cofilin 1. Also, miR-182-5p inhibited bladder tumor cell proliferation, migration, invasion and colony forming efficiency. Furthermore, xenograft tumor model assay showed that miR-182-5p plays a negative role in bladder cancer tumorigenesis abilities in vivo. Conclusion: Present results suggest that miR-182-5p could inhibit human bladder tumor growth by repressing Cofilin 1 expression. Our findings may provide a new horizon for exploring therapeutic target of bladder cancer.
- Subjects
BLADDER cancer; CANCER cell proliferation; CANCER; WESTERN immunoblotting; TUMOR growth; CANCER invasiveness
- Publication
Cancer Cell International, 2019, Vol 19, Issue 1, pN.PAG
- ISSN
1475-2867
- Publication type
Article
- DOI
10.1186/s12935-019-0758-5