We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Rhein exhibits antioxidative effects similar to Rhubarb in a rat model of traumatic brain injury.
- Authors
Xia Xu; Huiying Lv; Zian Xia; Rong Fan; Chunhu Zhang; Yang Wang; Dongsheng Wang
- Abstract
Background: The brain is secondarily harmed by pathological, physiological, and biological reactions that are caused by traumatic brain injury (TBI). Rhein, a significant composition of Rhubarb, is a well-known traditional Chinese treatment method and has a strong oxidation-resisting characteristic, but Rhein's mechanism remains unclear. Methods: This study aimed to identify Rhein in the brain tissues of TBI model of rats, and confirm whether Rhein induced an antioxidative effect similar to its parent medicine, Rhubarb. First, the ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was applied to identify Rhein in the brain tissue of the controlled cortical impact (CCI) rats after intra-gastric administration of Rhubarb. Further, for the purpose of calculating the oxidant stress of the CCI rats, the malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and glutathione disulfide (GSSG), as well as the proportion of glutathione (GSH)/GSSG were measured in the brain tissues. Results: The results showed that Rhein was absorbed in the brain tissues of CCI rats. Rhubarb and rhein elevated the SOD, CAT activities, GSH level, and GSH/GSSG ratio, and diminished the MDA and GSSG levels. Conclusion: The data demonstrated that Rhubarb and Rhein had the potential to be used as a neuroprotective drug for TBI, and that Rhein induced an antioxidative effect similar to its parent medicine, Rhubarb.
- Subjects
ANIMAL experimentation; ANTIOXIDANTS; BRAIN injuries; GLUTATHIONE; HERBAL medicine; HIGH performance liquid chromatography; LIQUID chromatography; CHINESE medicine; MOLECULAR structure; OXIDIZING agents; RATS; RESEARCH funding; RHUBARB; STATISTICS; SUPEROXIDE dismutase; MALONDIALDEHYDE; DATA analysis; OXIDATIVE stress; NEUROPROTECTIVE agents; DATA analysis software; DESCRIPTIVE statistics
- Publication
BMC Complementary & Alternative Medicine, 2017, Vol 17, p1
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/s12906-017-1655-x