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- Title
An alternative procedure to leukapheresis for peripheral hematopoietic progenitor cell collection in very‐low‐weight children: A single pediatric center experience.
- Authors
Becherucci, Valentina; Ermini, Stefano; Piccini, Luisa; Bisin, Silvia; Gori, Valentina; Gentile, Francesca; Ceccantini, Riccardo; De Rienzo, Elena; Bindi, Barbara; Pavan, Paola; Cunial, Vanessa; Allegro, Elisa; Brugnolo, Francesca; Maggio, Daniela; Calzolari, Daniela; Maccarelli, Erika; Galli, Silvia; Muricci, Sonia; Berchielli, Marco; Tintori, Veronica
- Abstract
Background: PBSC collection using a blood cell separator in very low weight patients can be frequently complicated by severe adverse effects and technical difficulties. Material and Methods: From March 2013 to January 2017, 14 PBSC collections were performed in 12 children weighing less than 10 kg, affected by different solid tumours. PBSC collection was performed with a "homemade" aseptically assembled circuit. The circuit is composed by a 150 mL collection bag connected with a 4 stopcock ramp, perfused with ACD. This circuit allows collection of a specific total blood amount from CVC, depending on CD34+/kg target. Results: Mean CD34+cell performance per collection was 9.3 × 106/kg. Tolerance to the procedure was very good as none of the patients experienced complications, with the exception of a patient who showed mild cyanosis and pallor after collection. Moreover, no bleeding or thrombotic complications have been observed. To date, 16 PBSC reinfusions have been performed in 7 children with a mean CD34+ cells viability of 98.1% ± 2.7 and mean WBC viability of 57% ± 10. Cell recovery after thawing was 87% ± 10.8. A rapid graft intake for both neutrophils and platelets, between day 7 and 20 after reinfusion was observed. Discussion The procedure of total blood collection without the use of a cell separator is feasible and allows a good PBSC collection without significant side effects in very low‐weight children. Moreover, this method could represent a valid and safe alternative to leukapheresis in patients where classic procedure could be difficult to apply.
- Subjects
LEUKAPHERESIS; PROGENITOR cells; BLOOD collection; BLOOD cells; CELL survival; COLLECTIONS; HIV-positive children; PEDIATRICIANS
- Publication
Journal of Clinical Apheresis, 2020, Vol 35, Issue 5, p406
- ISSN
0733-2459
- Publication type
Article
- DOI
10.1002/jca.21813