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- Title
Mycobacterium tuberculosis typing using Allele-specific oligonucleotide multiplex PCR (ASO–PCR) method.
- Authors
Shafipour, Maryam; Shirzad-Aski, Hesamaddin; Ghaemi, Ezzat Allah; Sohrabi, Ahmad; Babaii Kochaksaraei, Maya; Taziki, Masoume; Rahimi, Somayeh; Ghazvini, Kiarash; Baei, Basireh
- Abstract
Mycobacterium tuberculosis (M. tuberculosis) genotyping provides valuable information related to the origin and the evolution of the isolates. This study aimed to evaluate the applicability of single-nucleotide polymorphisms (SNPs) technique for lineages identification of M. tuberculosis and compare it with mycobacterial interspersed repetitive units–variable number of tandem repeats (MIRU–VNTR) method. The lineages of 162 clinically isolates were evaluated using six pair primers by Multiplex-PCR based on SNPs. Among 162 isolates, 70 (43.2%) isolates were lineage 4, following that 62 (38.3%) and 22 (13.6%) isolates were lineage 3 and 2, respectively. The method could not type 8 (4.9%) isolates. Moreover, we could identify 71 out of 79 unknown isolates resulted from the MIRU–VNTR method. The results showed that the SNP typing method has the potential to determine the lineages of M. tuberculosis as a rapid laboratory screening test.
- Subjects
MYCOBACTERIUM tuberculosis; TANDEM repeats; SINGLE nucleotide polymorphisms; POLYMERASE chain reaction; TUBERCULOSIS
- Publication
Current Microbiology, 2021, Vol 78, Issue 12, p4009
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-021-02659-7