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- Title
The inhibitory effect of disease-modifying anti-rheumatic drugs and steroids on gliostatin/platelet-derived endothelial cell growth factor production in human fibroblast-like synoviocytes.
- Authors
Kusabe, Takuma; Waguri-Nagaya, Yuko; Tanikawa, Tomohiro; Aoyama, Mineyoshi; Fukuoka, Muneyoshi; Kobayashi, Masaaki; Otsuka, Takanobu; Asai, Kiyofumi
- Abstract
Gliostatin/platelet-derived endothelial cell growth factor (GLS/PD-ECGF) is known to have both angiogenic and arthritogenic activities. The purpose of this study was to investigate whether disease-modifying anti-rheumatic drugs (DMARDs) and steroids are involved in the regulation of GLS expression. Fibroblast-like synoviocytes (FLSs) obtained from patients with rheumatoid arthritis (RA) were cultured and stimulated by interleukin (IL)-1β with or without DMARDs and steroids. The expression levels of GLS were determined using the reverse transcription-polymerase chain reaction and an ELISA. In cultured rheumatoid FLSs, the expression of GLS mRNA was significantly increased by stimulation with IL-1β. By contrast, GLS mRNA levels in IL-1β-stimulated FLSs were reduced by treatment with aurothioglucose (AuTG) and dexamethasone (DEX). These findings indicate that AuTG and DEX have anti-rheumatic activity, which is mediated via the suppression of GLS production. Neither methotrexate (MTX) nor sulfasalazine (SSZ) had a significant influence on GLS levels in our study.
- Subjects
PHARMACODYNAMICS; ANTIRHEUMATIC agents; STEROIDS; PLATELET-derived growth factor; BLOOD proteins; VASCULAR endothelial growth factors; RHEUMATOID arthritis; INTERLEUKIN-1
- Publication
Rheumatology International, 2005, Vol 25, Issue 8, p625
- ISSN
0172-8172
- Publication type
Article
- DOI
10.1007/s00296-005-0624-8