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- Title
Using time-lapse technology to explore vacuolization in embryos on Day 3 and Day 4.
- Authors
Zhang, Jingye; Zhong, Wanxia; Liu, Hui; Zhao, Haibin; Li, Mei; Ma, Shuiying; Wu, Keliang
- Abstract
<bold>Purpose: </bold>To investigate the occurrence and development state of embryo vacuoles between the 8-cell and morula stages, and to explore how vacuoles affected the development of embryos.<bold>Methods: </bold>A retrospective study of a cohort of 422 patients undergoing conventional in vitro fertilization or intracytoplasmic sperm injection. With the help of time-lapse imaging, the development processes and outcomes of good quality embryos with or without vacuoles were analyzed.<bold>Results: </bold>Vacuole positive embryos had significantly lower blastulation rate and good quality blastulation rate than vacuole negative embryos, p < 0.05. Compared to vacuole negative embryos, the number of best and good quality blastocysts was significantly reduced, while the number of fair and discarded ones was significantly increased, p < 0.05. The average starting time of vacuolization was 73.7 ± 9.3 h after insemination. The proportion of blastomeres affected by vacuoles was associated with embryonic developmental potential.<bold>Conclusions: </bold>Vacuolization on Day 3 and Day 4 was frequently observed and was detrimental to embryo development. The proportion of blastomeres affected by vacuoles may be an indicator of embryo developmental potential.
- Subjects
EMBRYOS; INTRACYTOPLASMIC sperm injection; BLASTOMERES; FERTILIZATION in vitro
- Publication
Archives of Gynecology & Obstetrics, 2019, Vol 299, Issue 3, p857
- ISSN
0932-0067
- Publication type
journal article
- DOI
10.1007/s00404-018-5008-x