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- Title
Virus purification by CsCl density gradient using general centrifugation.
- Authors
Nasukawa, Tadahiro; Uchiyama, Jumpei; Taharaguchi, Satoshi; Ota, Sumire; Ujihara, Takako; Matsuzaki, Shigenobu; Murakami, Hironobu; Mizukami, Keijirou; Sakaguchi, Masahiro
- Abstract
Virus purification by cesium chloride (CsCl) density gradient, which generally requires an expensive ultracentrifuge, is an essential technique in virology. Here, we optimized virus purification by CsCl density gradient using general centrifugation (40,000 × g, 2 h, 4 °C), which showed almost the same purification ability as conventional CsCl density gradient ultracentrifugation (100,000 × g, 1 h, 4 °C) using phages S13′ and φEF24C. Moreover, adenovirus strain JM1/1 was also successfully purified by this method. We suggest that general centrifugation can become a less costly alternative to ultracentrifugation for virus purification by CsCl densiy gradient and will thus encourage research in virology.
- Subjects
DENSITY gradient centrifugation; ULTRACENTRIFUGATION; ADENOVIRUSES; VIROLOGY -- Technique; BACTERIOPHAGES
- Publication
Archives of Virology, 2017, Vol 162, Issue 11, p3523
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-017-3513-z