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- Title
Isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from Jiangsu Province, China.
- Authors
Zhang, Hewei; Zhang, Zhuo; Wang, Yufei; Wang, Xin; Xia, Mingqi; Wu, Hua
- Abstract
In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 10 TCID/ml. By applying an 80 % nucleotide cutoff value and the RotaC automated genotyping tool, the Vp4 genotype of the new isolate was identified as P[7]. The Vp7 genotype was identified as G[9], lineage VI, and sublineage c. Experimentally infected piglets showed severe diarrhea symptoms 16-24 h post-inoculation, indicating that this new porcine rotavirus isolate is a pathogenic strain.
- Subjects
JIANGSU Sheng (China); ROTAVIRUS diseases; PATHOGENIC viruses; DIAGNOSIS of diarrhea; SYMPTOMS; GENOTYPES; LABORATORY swine
- Publication
Archives of Virology, 2015, Vol 160, Issue 5, p1333
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-015-2347-9