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- Title
Co-expression of multiple target proteins in plants from a tobacco mosaic virus vector using a combination of homologous and heterologous subgenomic promoters.
- Authors
Roy, Gourgopal; Weisburg, Sangeetha; Foy, Kelly; Rabindran, Shailaja; Mett, Vadim; Yusibov, Vidadi
- Abstract
To co-express multiple target proteins, we engineered a single-component chimeric tobacco mosaic virus (TMV)-based vector containing homologous and heterologous capsid protein subgenomic RNA promoters. Delivery of this vector into Nicotiana benthamiana plants via agroinfiltration resulted in co-expression of two reporter genes within a single cell. Furthermore, co-expression of a host-specific antisense RNA or a silencing suppressor protein from this vector augmented the accumulation of green fluorescent protein or a vaccine antigen, hemagglutinin from avian influenza virus A/Vietnam/1194/04. These findings suggest that this chimeric vector utilizing the homologous and heterologous subgenomic TMV promoters has a potential for high-level production of multiple therapeutic proteins including monoclonal antibodies.
- Subjects
TOBACCO mosaic virus; PROMOTERS (Genetics); NICOTIANA benthamiana; GENES; ANTISENSE RNA; HEMAGGLUTININ; PLANT proteins
- Publication
Archives of Virology, 2011, Vol 156, Issue 11, p2057
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-011-1069-x