We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Sequences of polycythemia-type Friend spleen focus-forming virus in clone-745-derived mouse erythroleukemia cells.
- Authors
Scher, William; Jing, Yongkui; Lu, Min; Bishop, David F.; Scher, Barbara M.
- Abstract
Friend leukemia virus complex consists of a replication-competent virus plus one of two replication-incompetent viruses, spleen focus-forming virus anemia virus or spleen focus-forming virus polycythemia virus. The replication-incompetent viruses induce rapid malignant transformation of erythroid precursor cells. Transformed cell lines from mice infected with the complex can be induced to undergo erythrodifferentiation in vitro. However, lines containing the anemia-type virus require erythropoietin and another agent such as dimethyl sulfoxide for optimal erythrodifferentiation, whereas those containing the polycythemia-type virus do not require or respond to erythropoietin. Mice infected with the original Friend virus isolates were anemic, so sub-lines derived from these mice should be erythropoietin-dependent for induction of erythrodifferentiation. However, many of the widely studied sub-lines are erythropoietin-independent. In order to clarify this apparent anomaly, the genomes of viruses present in two commonly used erythropoietin-independent sub-lines were sequenced. Sequence analysis demonstrates that they contain the polycythemia-type virus and not the anemia-type virus.
- Subjects
VIRAL replication; FRIEND virus; ERYTHROPOIETIN; DIMETHYL sulfoxide; POLYCYTHEMIA vera; LABORATORY mice; MICROBIAL differentiation
- Publication
Archives of Virology, 2009, Vol 154, Issue 5, p895
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-009-0372-2