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- Title
Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathway.
- Authors
Berenjabad, Nesa Janamo; Nejati, Vahid; Rezaie, Jafar
- Abstract
Background: Many attempts are used to discover mechanisms driving impaired angiogenesis in age-related diseases. Angiogenesis is highly regulated by different signaling pathways. Here, we investigated the angiogenesis potential of human endothelial cells (ECs) upon exposure to hydrogen peroxide (H2O2), a cellular senescent factor. Results: Data showed that the wound healing rate of HUVECs decreased upon incubation with H2O2 (P < 0.05). LOX activity and NO production were decreased in H2O2 treated cells (P < 0.05). Expression of miR-126 and VEGFR-2 up-regulated, while expression of miR-373 and HSP-70 up = regulated in H2O2 -induced cells (P < 0.05). In addition, we found that protein levels of p-Akt-1, VCAM-1, MMP-9, and IL-6 decreased in treated cells (P < 0.05). Conclusions: Our data showed that H2O2 reduced the angiogenic response of HUVECs in vitro, which may be due to impairment of the VEGFR-2 signaling pathway.
- Subjects
HYDROGEN peroxide; ENDOTHELIAL cells; CELLULAR signal transduction; WOUND healing; CELLULAR aging
- Publication
BMC Molecular & Cell Biology, 2022, Vol 23, Issue 1, p1
- ISSN
2661-8850
- Publication type
Article
- DOI
10.1186/s12860-022-00435-4