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- Title
Gene silencing in mammals by small interfering RNAs.
- Authors
McManus, Michael T.; Sharp, Phillip A.
- Abstract
Among the 3 billion base pairs of the human genome, there are ∼30,000–40,000 protein-coding genes, but the function of at least half of them remains unknown. A new tool — short interfering RNAs (siRNAs) — has now been developed for systematically deciphering the functions and interactions of these thousands of genes. siRNAs are an intermediate of RNA interference, the process by which double-stranded RNA silences homologous genes. Although the use of siRNAs to silence genes in vertebrate cells was only reported a year ago, the emerging literature indicates that most vertebrate genes can be studied with this technology.
- Subjects
HUMAN genome; RNA
- Publication
Nature Reviews Genetics, 2002, Vol 3, Issue 10, p737
- ISSN
1471-0056
- Publication type
Article
- DOI
10.1038/nrg908