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- Title
A Retrospective Whole-Genome Sequencing Analysis of Carbapenem and Colistin-Resistant Klebsiella pneumoniae Nosocomial Strains Isolated during an MDR Surveillance Program.
- Authors
Gentile, Bernardina; Grottola, Antonella; Orlando, Gabriella; Fregni Serpini, Giulia; Venturelli, Claudia; Meschiari, Marianna; Anselmo, Anna; Fillo, Silvia; Fortunato, Antonella; Lista, Florigio; Pecorari, Monica; Mussini, Cristina
- Abstract
Multidrug-resistant Klebsiella pneumoniae (MDR Kp), in particular carbapenem-resistant Kp (CR-Kp), has become endemic in Italy, where alarming data have been reported on the spread of colistin-resistant CR-Kp (CRCR-Kp). During the period 2013–2014, 27 CRCR-Kp nosocomial strains were isolated within the Modena University Hospital Policlinico (MUHP) multidrug resistance surveillance program. We retrospectively investigated these isolates by whole-genome sequencing (WGS) analysis of the resistome, virulome, plasmid content, and core single nucleotide polymorphisms (cSNPs) in order to gain insights into their molecular epidemiology. The in silico WGS analysis of the resistome revealed the presence of genes, such as blaKPC, related to the phenotypically detected resistances to carbapenems. Concerning colistin resistance, the plasmidic genes mcr1–9 were not detected, while known and new genetic variations in mgrB, phoQ, and pmrB were found. The virulome profile revealed the presence of type-3 fimbriae, capsular polysaccharide, and iron acquisition system genes. The detected plasmid replicons were classified as IncFIB(pQil), IncFIB(K), ColRNAI, IncX3, and IncFII(K) types. The cSNPs genotyping was consistent with the multi locus sequence typing (MLST) and with the distribution of mutations related to colistin resistance genes. In a nosocomial drug resistance surveillance program, WGS proved to be a useful tool for elucidating the spread dynamics of CRCR-Kp nosocomial strains and could help to limit their diffusion.
- Subjects
ITALY; KLEBSIELLA pneumoniae; SEQUENCE analysis; SINGLE nucleotide polymorphisms; MULTIDRUG resistance; DRUG utilization; MOLECULAR epidemiology
- Publication
Antibiotics (2079-6382), 2020, Vol 9, Issue 5, p246
- ISSN
2079-6382
- Publication type
Article
- DOI
10.3390/antibiotics9050246