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- Title
TBHQ Alleviates Particulate Matter-Induced Pyroptosis in Human Nasal Epithelial Cells.
- Authors
Kim, Ji-Sun; Choi, Hyunsu; Oh, Jeong-Min; Kim, Sung Won; Kim, Soo Whan; Kim, Byung Guk; Cho, Jin Hee; Lee, Joohyung; Lee, Dong Chang
- Abstract
Pyroptosis represents a type of cell death mechanism notable for its cell membrane disruption and the subsequent release of proinflammatory cytokines. The Nod-like receptor family pyrin domain containing inflammasome 3 (NLRP3) plays a critical role in the pyroptosis mechanism associated with various diseases resulting from particulate matter (PM) exposure. Tert-butylhydroquinone (tBHQ) is a synthetic antioxidant commonly used in a variety of foods and products. The aim of this study is to examine the potential of tBHQ as a therapeutic agent for managing sinonasal diseases induced by PM exposure. The occurrence of NLRP3 inflammasome-dependent pyroptosis in RPMI 2650 cells treated with PM < 4 µm in size was confirmed using Western blot analysis and enzyme-linked immunosorbent assay results for the pyroptosis metabolites IL-1β and IL-18. In addition, the inhibitory effect of tBHQ on PM-induced pyroptosis was confirmed using Western blot and immunofluorescence techniques. The inhibition of tBHQ-mediated pyroptosis was abolished upon nuclear factor erythroid 2-related factor 2 (Nrf2) knockdown, indicating its involvement in the antioxidant mechanism. tBHQ showed potential as a therapeutic agent for sinonasal diseases induced by PM because NLRP3 inflammasome activation was effectively suppressed via the Nrf2 pathway.
- Subjects
NUCLEAR factor E2 related factor; EPITHELIAL cells; PYROPTOSIS; CELL membranes; NASAL mucosa
- Publication
Toxics, 2024, Vol 12, Issue 6, p407
- ISSN
2305-6304
- Publication type
Article
- DOI
10.3390/toxics12060407