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- Title
The Effect of Remdesivir as an anti-COVID-19 Drug on Chicken Hepatocyte Enzymes; an in vitro Study.
- Authors
Mahdavi, Roya; Jonoush, Zahra Akbari; Ghafourian, Mehri; Khoshnam, Seyed Esmaeil; Dehbashi, Fereshteh Nezhad; Farzaneh, Maryam
- Abstract
Background and Aim: There is little in vitro evidence of potential hepatotoxicity of Remdesivir (RDV). Regarding the effective results of RDV in patients with COVID-19 and the impact of COVID-19 on liver function, we investigated the effects of RDV on the expression and activity of liver enzymes in chicken embryo-derived hepatocytes. Materials and Methods: In this in vitro study, 20 embryonated chicken eggs (stage X) were incubated (37.5 °C, 60-65% humidity) for 10 days (stage HH35). The hepatocytes were cultured in DMEM/F12+10% FBS medium and treated with four concentrations of RDV (2.00, 3.00, 4.00, and 5.00 µM). Serum levels of alanine (ALT) and aspartate (AST) aminotransferases were measured by enzyme-linked immunosorbent assay (ELISA), and gene expression was measured by quantitative realtime PCR (qPCR). Results: Each hepatocyte had a hexagonal structure with a large nucleus and nucleolus. In the PAS-positive cells with a pink color confirmed the glycogen content of hepatocytes. In the presence of 4 and 5 μM RDV resulted in a significant decrease in hepatocyte viability, up to 50% increase in the proportion of non-viable hepatocytes after 48 hr (P<0.001). Besides, the expression of both ALT and AST significantly increased after treatment with RDV (P<0.001). Our data demonstrated a significant increase in the function of both ALT and AST in the RDV+ group. Conclusion: We concluded that treatment with RDV led to increased expression and function of hepatocyte enzymes in vitro.
- Subjects
HEPATOTOXICOLOGY -- Risk factors; IN vitro studies; EGGS; DRUG efficacy; COVID-19; POULTRY; ANIMAL experimentation; SERUM; ANTIVIRAL agents; TISSUE viability; GENE expression; RISK assessment; TREATMENT effectiveness; ALANINE; ENZYMES; ENZYME-linked immunosorbent assay; LIVER cells; AMINOTRANSFERASES; POLYMERASE chain reaction; ASPARTATE aminotransferase
- Publication
Iranian Journal of Medical Microbiology, 2023, Vol 17, Issue 3, p309
- ISSN
1735-8612
- Publication type
Article
- DOI
10.30699/ijmm.17.3.309