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- Title
Efficacy study of allogeneic Mesenchymal Stem Cell Therapy for the treatment of Metabolic Syndrome in Animal Model.
- Authors
Lokanathan, Yogeswaran; Chan Man Lung, Alvin; Ng Min Hwei, Angela; Yunus, Mohd Heikal Mohd; Idrus, Ruszymah Hj; Law Jia Xian; Yazid, Muhammad Da'in; Chin Kok Yong; Shamsuddin, Sharen Aini; Yusof, Rafizul Mohd; Bin Hassan, Muhammad Najib Fathi; Ng See Nguan; Koh, Benson
- Abstract
Introduction: Recently, mesenchymal stem cells (MSCs) therapy have been proposed as a novel treatment for Metabolic Syndrome (MetS). The current study aimed to determine the efficacy of MSC IV injection in the rodent model of metabolic syndrome to alleviate the diseases associated with MetS. Methods: After 16 weeks of High Fat High Fructose diet, all animals were successfully induced with hypertension, hyperglycemia and hypertriglyceridemia. The animals were then intravenously administered with saline as control or pre-determined low-dose and highdose WJ-MSCs. Throughout the 12-week study, animals were evaluated for physical measurements, blood analysis, whole-body composition and cardiovascular function. During the terminal stages, necropsy and histopathology were performed. Results: The hypertension, hyperglycemia and hypertriglyceridemia levels were varied among the animals. No statistically significant differences (P<0.05) between animals from the treatment and control groups. However, we confirmed that different sub-clusters of MetS, such as animals with diabetes, obesity or high blood pressure, were manifested, similar to our pilot study to optimize the diet-induced MetS protocol. Therefore, the data were reassessed through individual or sub-grouped conditions of MetS, which provided a more reliable interpretation of our cell therapy. The WJ-MSCs showed a strong resolution of hemodynamic indices. It had also temporarily relieved excess adiposity, osteo-degenerative factors, and sudden weight loss from chronic insulin resistance. Despite that, the histopathology of liver and lungs in WJ-MSC treated groups showed physical improvements in a dose-dependent manner. Conclusion: WJ-MSCs transiently preserved the health status of MetS animals, but single dosage, unchanged diet, and sedentary factors may have limited further recovery. Beyond the scope of this study, we hypothesize that implementing multiple doses or co-therapy (e.g., exercise output or caloric management) could unlock the maximum curative abilities of WJ-MSCs.
- Subjects
MESENCHYMAL stem cells; STEM cell treatment; METABOLIC syndrome; HIGH-fat diet; ANIMAL models in research; CURATIVE medicine; HUMAN body composition
- Publication
Malaysian Journal of Medicine & Health Sciences, 2022, Vol 18, p13
- ISSN
1675-8544
- Publication type
Abstract