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- Title
Basophils and mast cells are crucial for reactions due to epicutaneous sensitization to ovalbumin.
- Authors
Yu, Rie; Igawa, Ken; Handa, Yutaro; Munetsugu, Takichi; Satoh, Takahiro; Yokozeki, Hiroo
- Abstract
The prevalence of food allergies worldwide has increased recently. Epicutaneous sensitization to antigen could be a method to study food allergy. To clarify the mechanisms of food allergy, we established a mouse model of epicutaneous sensitization using ovalbumin ( OVA). BALB/c mice were sensitized by three-time application of OVA to tape-stripped skin (1-week sensitization at 2-week intervals) and oral challenge of OVA undertaken. Rectal temperature was monitored. Blood and tissue (skin and jejunum) of challenged mice were taken. Numbers of mast cells ( MCs) and basophils were counted. Serum and/or tissue levels of OVA -specific IgE and IgG antibodies and several cytokines were measured using enzyme-linked immunoassay kits. MC and basophil depletion experiments were undertaken. In OVA/epicutaneous-sensitized and orally challenged mice, systemic anaphylaxis (as evidenced by reduced rectal temperature) was observed. Levels of OVA-specific IgE and IgG antibodies were increased in these mice, as were increased number of MCs and basophils. Serum levels of MC protease 1 were increased significantly. Basophil and MC depletion experiments revealed that they both participate in reactions. Increased production of thymic stromal lymphopoietin ( TSLP) at skin sites of OVA sensitization was noted. We speculate that TSLP produced from epidermal cells during antigen sensitization can enable basophils to promote a T helper (Th)2 immune reaction, leading to and systemic anaphylaxis by antigen-specific IgE-bearing MCs. This TSLP-basophils- MC axis could be a novel therapeutic target against food allergy.
- Subjects
FOOD allergy; BASOPHIL physiology; MAST cell physiology; OVALBUMINS; ENZYME-linked immunosorbent assay; THYMIC stromal lymphopoietin
- Publication
Experimental Dermatology, 2017, Vol 26, Issue 9, p778
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/exd.13279