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- Title
Modulation of bullous pemphigoid antigen gene expression by γ-interferon in cultured keratinocytes.
- Authors
Sugita, Y.; Nagatani, T.; Ikezawa, Z.; Nomura, K.; Watanabe, Y.; Uitto, J.; Nakajima, H.
- Abstract
Bullous pemphigoid (BP) is characterized hy the production of autoantibodies against BP antigens, γ-interferon (γ-IFN), a T-cell lymphokine, is known to enhance the expression of several cell-surface proteins. In this study, keratinocytes were cultured in the presence of γ-IFN. the expression of BF antigen protein was examined by flow cytometry and BP antigen messenger RNA (mRNA) (encoding 230-kDa protein) was quantified by slot-blot hybridization. The results indicated that BP antigen gene expression by keratinocytes was upregulated by γ-IFN. This enhancement of gene expression was detected at both the protein and mRNA level, suggesting pretranslational regulation. These results imply the involvement of not only humoral immunity but also cell-mediated immunity in the development of BP.
- Subjects
AUTOANTIBODIES; ANTIGENS; GENE expression; PROTEINS; KERATINOCYTES; MESSENGER RNA
- Publication
British Journal of Dermatology, 1992, Vol 126, Issue 5, p468
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1111/j.1365-2133.1992.tb11820.x