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- Title
Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections.
- Authors
Okba, Nisreen M. A.; Raj, V. Stalin; Widjaja, Ivy; GeurtsvanKessel, Corine H.; de Bruin, Erwin; Chandler, Felicity D.; Wan Beom Park; Nam-Joong Kim; Farag, Elmoubasher A. B. A.; Al-Hajri, Mohammed; Bosch, Berend-Jan; Myoung-don Oh; Koopmans, Marion P. G.; Reusken, Chantal B. E. M.; Haagmans, Bart L.; Park, Wan Beom; Kim, Nam-Joong; Oh, Myoung-Don
- Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed cases has been reported. We found that a MERS-CoV spike S1 protein-based ELISA, routinely used in surveillance studies, showed low sensitivity in detecting infections among PCR-confirmed patients with mild clinical symptoms and cross-reactivity of human coronavirus OC43-positive serum samples. Using in-house S1 ELISA and protein microarray, we demonstrate that most PCR-confirmed MERS-CoV case-patients with mild infections seroconverted; nonetheless, some of these samples did not have detectable levels of virus-neutralizing antibodies. The use of a sensitive and specific serologic S1-based assay can be instrumental in the accurate estimation of MERS-CoV prevalence.
- Subjects
COMPARATIVE studies; CORONAVIRUS diseases; ENZYME-linked immunosorbent assay; IMMUNOGLOBULINS; RESEARCH methodology; MEDICAL cooperation; POLYMERASE chain reaction; RESEARCH; TIME; VIRAL antibodies; EVALUATION research; SEVERITY of illness index; REVERSE transcriptase polymerase chain reaction; ANTIBODY formation; MERS coronavirus
- Publication
Emerging Infectious Diseases, 2019, Vol 25, Issue 10, p1868
- ISSN
1080-6040
- Publication type
journal article
- DOI
10.3201/eid2510.190051