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- Title
Preanalytical stability of SARS-CoV-2 anti-nucleocapsid antibodies.
- Authors
Niedrist, Tobias; Kriegl, Lisa; Zurl, Christoph J.; Schmidt, Felix; Perkmann-Nagele, Nicole; Mucher, Patrick; Repl, Manuela; Flieder, Ines; Radakovics, Astrid; Sieghart, Daniela; Radner, Helga; Aletaha, Daniel; Binder, Christoph J.; Gülly, Christian; Krause, Robert; Herrmann, Markus; Wagner, Oswald F.; Perkmann, Thomas; Haslacher, Helmuth
- Abstract
Anti-nucleocapsid (NC) antibodies are produced in response to SARS-CoV-2 infection. Therefore, they are well suited for the detection of a previous infection. Especially in the case of seroprevalence studies or during the evaluation of a novel in-vitro diagnostic test, samples have been stored at <−70 °C (short- and long-term) or 2–10 °C (short-term) before analysis. This study aimed to assess the impact of different storage conditions relevant to routine biobanking on anti-NC antibodies. The preanalytical impact of short-term storage (84 [58–98] days) on <−70 °C and for 14 days at 2–10 °C was evaluated using samples from 111 donors of the MedUni Vienna Biobank. Long-term effects (443 [409–468] days) were assessed using 208 samples from Biobank Graz and 49 samples from Biobank Vienna. Anti-Nucleocapsid antibodies were measured employing electrochemiluminescence assays (Roche Anti-SARS-CoV-2). After short-term storage, the observed changes did not exceed the extent that could be explained by analytical variability. In contrast, results after long-term storage were approximately 20% higher and seemed to increase with storage duration. This effect was independent of the biobank from which the samples were obtained. Accordingly, the sensitivity increased from 92.6 to 95.3% (p=0.008). However, comparisons with data from Anti-Spike protein assays, where these deviations were not apparent, suggest that this deviation could also be explained by the analytical variability of the qualitative Anti-NC assay. Results from anti-NC antibodies are stable during short-term storage at <−70 °C and 2–10 °C. After long-term storage, a slight increase in sensitivity could not be ruled out.
- Subjects
SARS-CoV-2; IMMUNOGLOBULINS; ELECTROCHEMILUMINESCENCE
- Publication
Clinical Chemistry & Laboratory Medicine, 2023, Vol 61, Issue 2, p332
- ISSN
1434-6621
- Publication type
Article
- DOI
10.1515/cclm-2022-0875