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- Title
Performance evaluation of Sysmex XN hematology analyzer in umbilical cord blood: a comparison study with Sysmex XE-2100.
- Authors
Kim, Hanah; Hur, Mina; Choi, Sang-Gyeu; Moon, Hee-Won; Yun, Yeo-Min; Hwang, Han Sung; Kwon, Han Sung; Sohn, In Sook
- Abstract
Background: The Sysmex XN (XN) modular system (Sysmex, Kobe, Japan) is a new automated hematology analyzer equipped with different principles from its previous version, Sysmex XE-2100. We compared the performances of Sysmex XN and XE-2100 in umbilical cord blood (CB) specimens. Methods: In 160 CB specimens, complete blood count (CBC) parameters and white blood cells (WBC) differentials were compared between the two analyzers. Their flagging performances for blasts, abnormal/atypical lymphocytes, immature granulocytes and/or left-shift (IG), and nucleated red blood cells (NRBC) counts were compared with manual counts. For the blast flagging, Q values by Sysmex XN were further compared with manual slide review. Results: Sysmex XN and XE-2100 showed high or very high correlations for most CBC parameters but variable correlations for WBC differentials. Compared with XE-2100, XN showed significantly different flagging performances for blasts, abnormal/atypical lymphocytes, and IG. The flagging efficiency for blasts was significantly better on Sysmex XN than on XE-2100 (85.0% vs. 38.8%): Sysmex XN showed a remarkably increased specificity of blast flag, compromising its sensitivity of blast flag. Among the 24 specimens with blasts (range, 0.5%-1.5%), only one (4.2%) showed a positive Q value. Conclusions: This study highlighted the remarkable differences of flagging performances between Sysmex XN and XE-2100 in CB specimens. The Sysmex XN modular system seems to be a suitable and practical option for the CB specimens used for hematopoietic stem cell transplantation as well as for the specimens from neonates.
- Subjects
HEMATOLOGY; CORD blood; BLOOD cells; LYMPHOCYTES; HEMATOPOIETIC stem cell transplantation; EQUIPMENT &; supplies
- Publication
Clinical Chemistry & Laboratory Medicine, 2014, Vol 52, Issue 12, p1771
- ISSN
1434-6621
- Publication type
Article
- DOI
10.1515/cclm-2014-0392