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- Title
Tyramide Signal Amplification: An Enhanced Method for Immunohistochemistry on Methyl-Methacrylate-Embedded Bone Marrow Trephine Sections.
- Authors
Gaiser, Timo; Bernhards, Joachim
- Abstract
The detection of cellular antigens in bone marrow sections depends on the method of embedding, the nature of antigen and antibody, antigen retrieval techniques and the sensitivity of the immunohistochemical method. This study evaluated a fluorescyl-tyramide-enhanced immunostaining method on methyl-methacrylate-embedded bone marrow trephine biopsies. Compared with the standard immunostaining technique, fluorescyl-tyramide-enhanced immunostaining showed a superior signal intensity on methyl- methacrylate-embedded bone marrow trephines. Thus, the fluorescyl-tyramide-based immunostaining method, while retaining specificity, is highly sensitive and provides optimal staining results for a broad panel of antibodies commonly used for the routine diagnosis and classification of hematological disorders. Copyright © 2007 S. Karger AG, Basel
- Subjects
BONE marrow; IMMUNOHISTOCHEMISTRY; IMMUNE system; IMMUNOGLOBULINS; METHYL methacrylate; CLINICAL pathology
- Publication
Acta Haematologica, 2007, Vol 117, Issue 2, p122
- ISSN
0001-5792
- Publication type
Article
- DOI
10.1159/000097458