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- Title
RESEARCH CONCERNING THE BEHAVIOR OF THE PRUNUS SERRULATA "KANZAN" VARIETY IN THE PROCESS OF IN VITRO REPRODUCTION.
- Authors
DUTA, Magdalena
- Abstract
The research that was effectuated has had for a purpose the study of the Prunus serrulata "Kanzan" variety behavior during the phases of initiation and in vitro reproduction. In view of the initiation of the culture there have been drawn explants, in the vegetative repose phase. The nutritive media used have had a different content of substances in relation to the in vitro culture phase. For the initiation of cultures the influence of benzylaminopurine was tested, in concentrations of 0,5; 1; 1,5 mg/l. During the microreproduction phase on a constant level of indolebutyric acid and gibberellic acid (0,1 mg/l), was tested the influence of benzylaminopurine (1; 1,4; 2 mg/l). In the room designated for growth were assured controlled temperature conditions (22 - 24°C), photoperiod (12 - 16 hours) and luminosity of 3500 lucs. The observations and registered data emphasized the influence of BAP over the growth and microreproduction of explants. During the initiation phase of cultures were obtained 95% explants grown with normal aspect. The rate of reproduction increased from 10,6 to 20,3 micro young shoots/explants depending on the increase of cytochinine concentration from 1 to 2 mg/l. The reduction of the photoperiod to 12 hours determined the decrease of the percentage of plants growth and of the rate of reproduction.
- Subjects
PRUNUS; PLANT physiology; PLANT development; PLANT growth-promoting rhizobacteria; GIBBERELLIC acid; REJUVENESCENCE (Botany); TREE reproduction
- Publication
Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Horticulture, 2008, Vol 65, Issue 1, p84
- ISSN
1843-5254
- Publication type
Article