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- Title
Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals.
- Authors
Wenjing Tian; Heidi Qunhui Xie; Hualing Fu; Xinhui Pei; Bin Zhao
- Abstract
With the development of biotechnology, approaches based on antibodies, such as enzyme-linked immunosorbent assay (ELISA), active aryl hydrocarbon immunoassay (Ah-I) and other multi-analyte immunoassays, have been utilized as alternatives to the conventional techniques based on gas chromatography and mass spectroscopy for the analysis of dioxin and dioxin-like compounds in environmental and biological samples. These screening methods have been verified as rapid, simple and cost-effective. This paper provides an overview on the development and application of antibody-based approaches, such as ELISA, Ah-I, and multi-analyte immunoassays, covering the sample extraction and cleanup, antigen design, antibody preparation and immunoanalysis. However, in order to meet the requirements for on-site fast detection and relative quantification of dioxins in the environment, further optimization is needed to make these immuno-analytical methods more sensitive and easy to use.
- Subjects
DIOXINS; CHEMICAL detectors; BIOTECHNOLOGY; ENZYME-linked immunosorbent assay; IMMUNOASSAY; GAS chromatography/Mass spectrometry (GC-MS); COST effectiveness
- Publication
Sensors (14248220), 2012, Vol 12, Issue 12, p16710
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s121216710