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- Title
RNF20 Functions as a Transcriptional Coactivator for PPARγ by Promoting NCoR1 Degradation in Adipocytes.
- Authors
Yong Geun Jeon; Jae Ho Lee; Yul Ji; Jee Hyung Sohn; Dabin Lee; Dong Wook Kim; Seul Gi Yoon; Kyung Cheul Shin; Jeu Park; Je Kyung Seong; Je-Yoel Cho; Sung Sik Choe; Jae Bum Kim; Jeon, Yong Geun; Lee, Jae Ho; Ji, Yul; Sohn, Jee Hyung; Lee, Dabin; Kim, Dong Wook; Yoon, Seul Gi
- Abstract
Adipose tissue is the key organ coordinating whole-body energy homeostasis. Although it has been reported that ring finger protein 20 (RNF20) regulates lipid metabolism in the liver and kidney, the roles of RNF20 in adipose tissue have not been explored. Here, we demonstrate that RNF20 promotes adipogenesis by potentiating the transcriptional activity of peroxisome proliferator-activated receptor-γ (PPARγ). Under normal chow diet feeding, Rnf20 defective (Rnf20+/- ) mice exhibited reduced fat mass with smaller adipocytes compared with wild-type littermates. In addition, high-fat diet-fed Rnf20+/- mice alleviated systemic insulin resistance accompanied by a reduced expansion of fat tissue. Quantitative proteomic analyses revealed significantly decreased levels of PPARγ target proteins in adipose tissue of Rnf20+/- mice. Mechanistically, RNF20 promoted proteasomal degradation of nuclear corepressor 1 (NCoR1), which led to stimulation of the transcriptional activity of PPARγ. Collectively, these data suggest that RNF20-NCoR1 is a novel axis in adipocyte biology through fine-tuning the transcriptional activity of PPARγ.
- Subjects
FAT cells; ADIPOSE tissues; TISSUE expansion; INSULIN resistance; BIOLOGY; PROTEIN metabolism; ANIMAL experimentation; COMPARATIVE studies; ANIMAL nutrition; ENZYMES; EPITHELIAL cells; RESEARCH methodology; MEDICAL cooperation; METABOLISM; MICE; OBESITY; PROTEINS; RESEARCH; EVALUATION research
- Publication
Diabetes, 2020, Vol 69, Issue 1, p20
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/db19-0508