We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Deciphering the Biology of Circulating Tumor Cells through Single-Cell RNA Sequencing: Implications for Precision Medicine in Cancer.
- Authors
Orrapin, Santhasiri; Thongkumkoon, Patcharawadee; Udomruk, Sasimol; Moonmuang, Sutpirat; Sutthitthasakul, Songphon; Yongpitakwattana, Petlada; Pruksakorn, Dumnoensun; Chaiyawat, Parunya
- Abstract
Circulating tumor cells (CTCs) hold unique biological characteristics that directly involve them in hematogenous dissemination. Studying CTCs systematically is technically challenging due to their extreme rarity and heterogeneity and the lack of specific markers to specify metastasis-initiating CTCs. With cutting-edge technology, single-cell RNA sequencing (scRNA-seq) provides insights into the biology of metastatic processes driven by CTCs. Transcriptomics analysis of single CTCs can decipher tumor heterogeneity and phenotypic plasticity for exploring promising novel therapeutic targets. The integrated approach provides a perspective on the mechanisms underlying tumor development and interrogates CTCs interactions with other blood cell types, particularly those of the immune system. This review aims to comprehensively describe the current study on CTC transcriptomic analysis through scRNA-seq technology. We emphasize the workflow for scRNA-seq analysis of CTCs, including enrichment, single cell isolation, and bioinformatic tools applied for this purpose. Furthermore, we elucidated the translational knowledge from the transcriptomic profile of individual CTCs and the biology of cancer metastasis for developing effective therapeutics through targeting key pathways in CTCs.
- Subjects
RNA sequencing; BIOLOGY; INDIVIDUALIZED medicine; CELL separation; PHENOTYPIC plasticity
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 15, p12337
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms241512337