We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
PPARGC1A Is a Moderator of Skeletal Muscle Development Regulated by miR-193b-3p.
- Authors
Ma, Manting; Cai, Bolin; Kong, Shaofen; Zhou, Zhen; Zhang, Jing; Zhang, Xiquan; Nie, Qinghua
- Abstract
Meat production performance is one of the most important factors in determining the economic value of poultry. Myofiber is the basic unit of skeletal muscle, and its physical and chemical properties determine the meat quality of livestock and poultry to a certain extent. Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) as a transcriptional coactivator has been found to be widely involved in a series of biological processes. However, PPARGC1A is still poorly understood in chickens. In this manuscript, we reported that PPARGC1A was highly expressed in slow-twitch myofibers. PPARGC1A facilitated mitochondrial biogenesis and regulated skeletal muscle metabolism by mediating the flux of glycolysis and the TCA cycle. Gain- and loss-of-function analyses revealed that PPARGC1A promoted intramuscular fatty acid oxidation, drove the transformation of fast-twitch to slow-twitch myofibers, and increased chicken skeletal muscle mass. Mechanistically, the expression level of PPARGC1A is regulated by miR-193b-3p. Our findings help to understand the genetic regulation of skeletal muscle development and provide a molecular basis for further research on the antagonism of skeletal muscle development and fat deposition in chickens.
- Subjects
PGC-1 protein; MUSCLE growth; SKELETAL muscle; FATTY acid oxidation
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 17, p9575
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23179575