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- Title
MicroRNA-137 Inhibits Cancer Progression by Targeting Del-1 in Triple-Negative Breast Cancer Cells.
- Authors
Lee, Soo Jung; Jeong, Jae-Hwan; Kang, Seung Hee; Kang, Jieun; Kim, Eun Ae; Lee, Jeeyeon; Jung, Jin Hyang; Park, Ho Yong; Chae, Yee Soo
- Abstract
MicroRNAs (miRNAs) can be used to target a variety of human malignancy by targeting their oncogenes or tumor suppressor genes. The developmental endothelial locus-1 (Del-1) might be under miRNA regulation. This study investigated microRNA-137 (miR-137) function and Del-1 expression in triple-negative breast cancer (TNBC) cells and tissues. Del-1 mRNA and miRNA-137 levels were determined via qRT-PCR in breast cancer cells (MDA-MB-231, MCF7, SK-BR3, and T-47D) and tissues from 30 patients with TNBC. The effects of miR-137 on cell proliferation, migration, and invasion were determined using MTT assays, wound healing, and Matrigel transwell assays. The luciferase reporter assay revealed direct binding of miR-137 to the 3′-UTR of Del-1. miR-137 inhibited cell proliferation, migration, and invasion of MDA-MB-231 cells. Among the 30 TNBC specimens, miR-137 was downregulated and Del-1 level in plasma was significantly elevated relative to normal controls. It is concluded that miR-137 regulates Del-1 expression in TNBC by directly binding to the Del-1 gene and cancer progression. The results implicate miR-137 as a new therapeutic biomarker for patients with TNBC.
- Subjects
TRIPLE-negative breast cancer; CANCER invasiveness; CANCER cells; TUMOR suppressor genes; CANCER genes
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 24, p6162
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20246162