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- Title
Increased -Carotene Production in Recombinant Escherichia coliHarboring an Engineered Isoprenoid Precursor Pathway with Mevalonate Addition.
- Authors
Sang-Hwal Yoon; Hye-Min Park; Ju-Eun Kim; Sook-Hee Lee; Myung-Suk Choi; Jae-Yean Kim; Deok-Kun Oh; Jay D. Keasling; Seon-Won Kim
- Abstract
When pT-LYCm4 containing lycopene synthetic genes was co-transformed with pSUcrtY or pSHcrtY containing crtYgene of Pantoea ananatis(P. ananatis) or Pantoea agglomerans(P. agglomerans), -carotene productions of 36 and 35 mg/L were obtained, respectively. No lycopene was detected in the -carotene production culture. pT-HB, constructed by addition of P. ananatis crtYgene into pT-LYCm4, was used for co-transformation with pSdxs and pSSN12Didi, which increased isopentenyl diphosphate and dimethylallyl diphosphate synthesis. -Carotene production significantly increased 1.5-fold (51 mg/L) with the amplification of the dxsgene through pSdxs and 4-fold (135 mg/L) with the mevalonate bottom pathway of pSSN12Didi in the presence of 3.3 mM mevalonate. The pT-DHB, constructed by integrating the dxsgene into pT-HB, was used for cotransformation of Escherichia coli(E. coli) harboring pSSN12Didi, resulting in -carotene production of 141 mg/L. Recombinant E. coliharboring pT-DHB and pSSN12Didi was used to maximize -carotene production by adjusting the available amounts of glycerol, a carbon source, and mevalonate, the precursor of the mevalonate bottom pathway. When recombinant E. coliwas given 16.5 mM mevalonate and 2.5% (w/v) glycerol, -carotene production of 503 mg/L in concentration and 49.3 mg/g DCW in content was obtained at 144 h, which was the highest level of carotenoid production in E. coliever reported in the literature.
- Subjects
LYCOPENE; CAROTENES; ESCHERICHIA coli; ISOPENTENOIDS
- Publication
Biotechnology Progress, 2007, Vol 23, Issue 3, p599
- ISSN
8756-7938
- Publication type
Article