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- Title
One-Component Styrene Monooxygenases: An Evolutionary View on a Rare Class of Flavoproteins.
- Authors
Tischler, Dirk; Gröning, Janosch; Kaschabek, Stefan; Schlömann, Michael
- Abstract
Styrene monooxygenases (SMOs) are catalysts for the enantioselective epoxidation of terminal alkenes. Most representatives comprise a reductase and a monooxygenase which are encoded by separate genes ( styA, styB). Only six presumed self-sufficient one-component SMOs ( styA2B) have previously been submitted to databases, and one has so far been characterized. StyA2B can be supported by another epoxidase (StyA1) encoded by styA1, a gene in direct neighborhood of styA2B. The present report describes the identification of a further styA1/ styA2B-like SMO, which was detected in Rhodococcus opacus MR11. Based on the initially available sequences of styA2B-type SMOs, primers directed at conserved sequences were designed and a 7,012-bp genomic fragment from strain MR11 was obtained after PCRs and subsequent genome walking. Six open reading frames (ORFs) were detected and compared to genomic fragments of strains comprising either two- or one-component SMOs. Among the proteins encoded by the ORFs, the monooxygenase StyA1/StyA2B showed the highest divergence on amino acid level when comparing proteins from different sources. That finding, a rare distribution of styA2B genes among bacteria, and the general observation of evolution from simple to complex systems indicate that one-component SMOs evolved from two-component ancestors. Analysis of gene products from styA/ styB- and styA1/ styA2B-like SMOs revealed that a fusion of styA/ styB to styA2B might have happened at least twice among microorganisms. This points to a convergent evolution of one-component SMOs.
- Subjects
STYRENE; MONOOXYGENASES; FLAVOPROTEINS; DATABASES; EPOXIDASES
- Publication
Applied Biochemistry & Biotechnology, 2012, Vol 167, Issue 5, p931
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-012-9659-y