We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Effect of α-tocopherol and N-acetylcysteine on benzoyl peroxide toxicity in human keratinocytes.
- Authors
Bellei, Elisa; Rota, Cristina; Bergamini, Stefania; Manfredini, Paolo; Albertazzi, Alberto; Tomasi, Aldo; Iannone, Anna
- Abstract
Benzoyl peroxide is a free-radical generating compound widely used in the polymer industry and also in pharmaceuticals as antimicrobial agent to treat acne. However, benzoyl peroxide causes irritation and contact dermatitis in about 1% of patients. Concern over the use of this compound is motivated by the demonstration that it can also act as skin tumor promoter in mice. In addition, benzoyl peroxide induces DNA strand breaks in many cells, including keratinocytes. Benzoyl peroxide toxicity is presumably mediated by the formation of reactive free radicals and by the consumption of intracellular antioxidants. In this work we investigated the effect of both the lipophilic antioxidant α-tocopherol and the hydrophilic thiol donor N-acetylcysteine (NAC) in human keratinocyte line HaCaT exposed to benzoyl peroxide. A protective effect against benzoyl peroxide cytotoxicity was achieved when cells were grown on a α-tocopherol layer. On the contrary, the addition of α-tocopherol dissolved in ethanol had a pro-oxidant effect, leading to an enhancement of benzoyl peroxide toxicity. Cytotoxicity was also reduced adding NAC to the culture medium; the presence of both NAC and α-tocopherol exerts a synergis- tic cytoprotection. © 2004 Wiley Periodicals, Inc. J Biochem Mol Toxicol 18:107-114, 2004; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20008
- Publication
Journal of Biochemical & Molecular Toxicology, 2004, Vol 18, Issue 2, p107
- ISSN
1095-6670
- Publication type
Article
- DOI
10.1002/jbt.20008