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- Title
A Role for Cell-Surface CSF-1 in Osteoblast-mediated Osteoclastogenesis.
- Authors
Yao, G.-Q.; Sun, B. H.; Weir, E. C.; Insogna, K. L.
- Abstract
CSF-1 is required for osteoblast-mediated osteoclast formation. Osteoblasts produce soluble (sCSF-1) and cell-surface forms of CSF-1 (also known as membrane-bound CSF-1, mCSF-1) but their individual contributions to osteoclastogenesis remain unclear. Using glutaraldehyde-fixed primary murine osteoblasts as a source of mCSF-1, osteoblasts from op/op mice as a source for other osteoblast-derived osteoclastogenic factors and murine bone marrow as a source of osteoclast progenitors, osteoclast-like cells (OCL) formation was observed after 7-9 days of co-culture. In contrast, no OCL formation occurred when mCSF-1 expressed by primary murine osteoblasts was blocked by CSF-1 antibody pretreatment or when op/op osteoblasts were substituted for primary murine osteoblasts in the co-culture system. Osteoclast formation was also significantly inhibited when murine primary osteoblasts were pretreated with an antisense phosphorothioate oligonucleotide against mCSF-1. Finally, mCSF-1 and sCSF-1 were synergistic in stimulating OCL formation. These data support the conclusion that mCSF-1 plays an important role in osteoblast-mediated osteoclastogenesis within the bone microenvironment.
- Subjects
CELL membranes; CEREBROSPINAL fluid proteins; OSTEOCLASTS; BONE marrow; BONE cells; IMMUNE system
- Publication
Calcified Tissue International, 2002, Vol 70, Issue 4, p339
- ISSN
0171-967X
- Publication type
Article
- DOI
10.1007/s00223-001-1079-x