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- Title
Association of Pneumococcal Carriage and Expression of Foxp3+ Regulatory T Cells and Th17 Cells in the Adenoids of Children.
- Authors
Jiang, Xiao-li; Zhang, Guang-li; Yang, Ting; Yang, Bao-hui; Wang, Li-jia; Wang, Qing-hong; Luo, Zheng-xiu; Liu, En-mei; Fu, Zhou
- Abstract
Background: Pneumococcal carriage in the nasopharynx is a primary means of transmission and a necessary prerequisite for pneumococcal disease. Objectives: We analyzed the relationship between expressions of Foxp3+ regulatory T (Treg) cells and Th17 cells, and pneumococcal carriage in the adenoids of children who were either positive or negative for pneumococci. Methods: We collected adenoidal tissue and nasopharyngeal swab samples from children undergoing an adenoidectomy. Adenoidal mononuclear cells were isolated, cultured and then stimulated with culture concentrated supernatant (CCS) obtained from a D39 bacterial strain. Results: Foxp3+ Treg cells were upregulated and Th17 cells were downregulated in populations of adenoidal mononuclear cells obtained from the pneumococcus-positive group. Following CCS stimulation, the increment in Foxp3+ Treg cells in the pneumococcus-positive group was significantly greater than that in the pneumococcus-negative group, while the increment in Th17 cells was less as compared to that in the pneumococcus-negative group. These results were consistent with variations in levels of Foxp3 mRNA and retinoic acid receptor-related orphan receptor-γt mRNA in adenoidal mononuclear cells. Levels of IL-17A and IL-6 in adenoid tissue were higher in the pneumococcus-negative group, and the levels of TGF-β in adenoid tissue were lower in the pneumococcus-negative group compared to the pneumococcus-positive group. Pneumococcal carriage in children was closely associated with the expressions of Foxp3+ Treg and Th17 cells in the adenoid. Conclusion: Upregulation of Foxp3+ Treg cells might downregulate the production of Th17 cells in the adenoid, resulting in decreased scavenging of Streptococcus pneumoniae and chronic pneumococcal carriage. © 2015 S. Karger AG, Basel
- Subjects
NASOPHARYNX microbiology; STREPTOCOCCAL diseases; RNA analysis; ADENOIDECTOMY; ADENOIDS; CARRIER state (Communicable diseases); CHI-squared test; GENE expression; RESEARCH funding; T-test (Statistics); DATA analysis software; DESCRIPTIVE statistics; MONONUCLEAR leukocytes; CHILDREN; INFECTIOUS disease transmission
- Publication
Respiration, 2015, Vol 90, Issue 1, p25
- ISSN
0025-7931
- Publication type
Article
- DOI
10.1159/000381724