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- Title
Isolation, Identification and Pathogenicity Analysis of Streptococcus suis Type 2.
- Authors
Zicheng MA; Yan LI; Jinyuan GU; Tao PENG; Zhaohu LIU; Hongyu WANG; Fanliang MENG; Fangkun WANG; Sidang LIU
- Abstract
[Objectives] This study aimed to investigate the pathogenicity, growth characteristics and drug resistance of Streptococcus suis type 2. [Methods] Bacterial isolation and identification, biochemical experiments, determination of growth curve and correlation curve between 0D(ai values and viable counts, drug susceptibility tests, pathogenicity analysis, and histopalhological observations were carried out. [Results] The Streptococcus strain isolated from infected pigs was identified as Streptococcus suis type 2, which was named TA01 strain. TA01 strain reached die growth peak at 6 -8 h post-incubation, and viable counts gradually declined after 8 li of inculwtion. The correlation equation between ODm values and viable counts is y =24.659x - 1.076 1, R2 =0.996 7. TA01 strain was sensitive to penicillin, erythromycin, florfenicol and oxacillin, and resistant to ciprofloxacin, polymyxin B and clindamycin. According to the results of pathogenicity analysis, all the mice in 3.6x109 c-fu/mouse group died within 48, and these dead mice exhibited acute pyaemia septiea. Based on the Heed-Muench formula, it was calculated that LD50 of TA01 strain was 1.137x108 cfu/mouse. Pathological examination showed obvious blue-stained bacteria clusters, accompanied by neutrophil infiltration. [Conclusions] TA01 strain was a virulent strain of Streptoa>ccus suis type 2. Compared with Streptococcus strains which were isolated and reported in China, TA01 strain exhibited strong virulence and rapid proliferation.
- Subjects
CHINA; STREPTOCOCCUS suis; POLYMYXIN B; CLINDAMYCIN; OXACILLIN; MICROBIAL virulence; DRUG resistance; ERYTHROMYCIN; ACTINOBACILLUS
- Publication
Agricultural Biotechnology (2164-4993), 2019, Vol 8, Issue 4, p64
- ISSN
2164-4993
- Publication type
Article