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- Title
Effect and Its Mechanism of Brassinolide-regulated Starch Metabolism in Rice Germination.
- Authors
Qianfeng Li; Jiawen Yu; Jun Lu; Min Xiong; Changquan Zhang; Qiaoquan Liu
- Abstract
The study was aimed to explore the effect and mechanism of brassinolide (BL) regulated starch metabolism in rice endosperm during seed germination. The radicle elongation of rice seeds treated with 1 (j,mol/L BL was inhibited during germination. The analysis on rice seeds with a Gf7S-fused promoter showed that BL had different regulatory effects on Wx, SBEI, and AGPS1. The effect of BL treatment on the physicochemical properties of rice starch was further investigated by scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. The results showed that the starch treated with BL maintained better crystallinity and orderly structure, indicating that during the germination process, the degradation of starch in endosperm was slow, which might be one of the reasons for the slow radicle growth in the early stages of seed germination. This study provided important clues for further analysis of the molecular mechanisms underlying BR-regulated rice seed germination.
- Subjects
BRASSINOLIDE; STARCH metabolism; GERMINATION; RICE breeding; ENDOSPERM; ELONGATION factors (Biochemistry)
- Publication
Agricultural Biotechnology (2164-4993), 2018, Vol 7, Issue 3, p10
- ISSN
2164-4993
- Publication type
Article