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- Title
One‐Pot Cascade Biotransformation for Efficient Synthesis of Benzyl Alcohol and Its Analogs.
- Authors
Liu, Lijun; Zhu, Yuling; Chen, Yufen; Chen, Huiyu; Fan, Cong; Mo, Qiwen; Yuan, Jifeng
- Abstract
Benzyl alcohol is a naturally occurring aromatic alcohol and has been widely used in the cosmetics and flavor/fragrance industries. The whole‐cell biotransformation for synthesis of benzyl alcohol directly from bio‐based L‐phenylalanine (L‐Phe) was herein explored using an artificial enzyme cascade in Escherichia coli. Benzaldehyde was first produced from L‐Phe via four heterologous enzymatic steps that comprises L‐amino acid deaminase (LAAD), hydroxymandelate synthase (HmaS), (S)‐mandelate dehydrogenase (SMDH) and benzoylformate decarboxylase (BFD). The subsequent reduction of benzaldehyde to benzyl alcohol was achieved by a broad substrate specificity phenylacetaldehyde reductase (PAR) from Solanum lycopersicum. We found the designed enzyme cascade could efficiently convert L‐Phe into benzyl alcohol with conversion above 99%. In addition, we also examined L‐tyrosine (L‐Tyr) and m‐fluoro‐phenylalanine (m‐f‐Phe) as substrates, the cascade biotransformation could also efficiently produce p‐hydroxybenzyl alcohol and m‐fluoro‐benzyl alcohol. In summary, the developed biocatalytic pathway has great potential to produce various high‐valued fine chemicals.
- Subjects
BENZYL alcohol; SYNTHETIC enzymes; TOMATOES; COSMETICS industry; BENZALDEHYDE; ESCHERICHIA coli
- Publication
Chemistry - An Asian Journal, 2020, Vol 15, Issue 7, p1018
- ISSN
1861-4728
- Publication type
Article
- DOI
10.1002/asia.201901680