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- Title
Osteoinduction by a collagen mineral composite combined with isologous bone marrow in a subcutaneous rat model.
- Authors
Alvis, Mark; Lalor, Peggy; Brown, Melissa K. C.; Thorn, Malahki R.; Block, Jon E.; Hornby, Samantha; Berg, Richard; Reddi, A. Hari
- Abstract
In a subcutaneous implant rat model, Collagraft alone (n=8), Collagraft plus isologous bone marrow (n=8), and marrow alone (n=8) were evaluated. Twelve rats were euthanized at 11 days and 12 at 21 days. Explants were evaluated histologically for evidence of bone formation, and osteogenic activity was determined by an assay for alkaline phosphatase. Histological bone formation was absent in all groups at 11 days. At 21 days, in the Collagraft alone and bone marrow alone groups, no bone induction was noted. In contrast, 21-day specimens from the Collagraft plus bone marrow group showed newly formed bone. Alkaline phosphatase activity was negligible (<0.05 units/mg) in all 11-day specimens. Activity in Collagraft plus bone marrow specimens at 21 days (0.38 units/mg) was significantly higher than any other group (P<.001 for all comparisons). This study demonstrates that Collagraft plus bone marrow is an osteoinductive matrix.
- Subjects
BONE marrow; BONE growth; SUBCUTANEOUS surgery; ALKALINE phosphatase; LABORATORY rats; BONE marrow physiology; COLLAGEN; BIOLOGICAL models; RESEARCH; ANIMAL experimentation; RESEARCH methodology; BONE substitutes; MEDICAL cooperation; EVALUATION research; RATS; COMPARATIVE studies; PHOSPHATES
- Publication
Orthopedics, 2003, Vol 26, Issue 1, p77
- ISSN
0147-7447
- Publication type
journal article
- DOI
10.3928/0147-7447-20030101-19