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- Title
Implementing mass-scale red cell genotyping at a blood center.
- Authors
Flegel, Willy A.; Gottschall, Jerome L.; Denomme, Gregory A.
- Abstract
<bold>Background: </bold>When problems with compatibility beyond ABO and D arise, currently transfusion services search their inventories and perform time-consuming serologic testing to locate antigen-negative blood. These clinically important blood group antigens can be detected reliably by red cell genotyping, which is a technology whereby DNA-based techniques are used to evaluate gene polymorphisms that determine the expression of blood group antigens. We introduced mass-scale genotyping and measured availability of genotyped blood.<bold>Study Design and Methods: </bold>All non-Caucasian donors qualified for genotyping along with donors who had a history of repeat donation. Mass-scale red cell genotyping, performed on an electronic interfaced open array platform, was implemented to screen blood donors for 32 single-nucleotide polymorphisms that predicted 42 blood group antigens. Genotype screening results were confirmed by phenotyping, when needed for antigen-negative transfusion, before release of the red blood cell (RBC) unit.<bold>Results: </bold>Approximately 22,000 donors were red cell genotyped within 4 months and a total of 43,066 donors in 4 years. There were 463 discordances (0.52% of 89,596 genotypes with a phenotype). Among the 307 resolved discordances, approximate equal numbers represented historical serologic or genotyping discrepancies (n = 151 and n = 156, respectively). In the final year of the study, a mean of 29% of the daily inventory had a genotype.<bold>Conclusions: </bold>Red cell genotyping of blood donors using an electronic interface created a large and stable supply of RBC units with historical genotypes. The database served the needs of antigen-negative blood requests for a large regional blood center and allowed us to abandon screening by serology.
- Subjects
BLOOD transfusion; ABO blood group system; SEROLOGY; MEDICAL centers; ANTIGENS; GENETIC polymorphisms; GENE expression; ERYTHROCYTE metabolism; ERYTHROCYTES; BLOOD donors; BLOOD groups; POLYMERASE chain reaction; RESEARCH funding; PHENOTYPES; BLOOD grouping &; crossmatching; GENOTYPES
- Publication
Transfusion, 2015, Vol 55, Issue 11, p2610
- ISSN
0041-1132
- Publication type
journal article
- DOI
10.1111/trf.13168