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- Title
Development of an Indirect Dot-PPA-ELISA using glutamate dehydrogenase as a diagnostic antigen for the rapid and specific detection of Streptococcus suis and its application to clinical specimens.
- Authors
Xia, Xiao-Jing; Wang, Lei; Shen, Zhi-Qiang; Qin, Wanhai; Hu, Jianhe; Jiang, Shi-Jin; Li, Shu-Guang
- Abstract
Streptococcus suis is an important zoonotic pathogen causing infections in pigs and humans. Bacterial surface-related proteins are often explored as potential vaccine candidates and diagnostic antigens. In the present study, glutamate dehydrogenase, a highly conserved immunogenic extracellular protein, was used to establish a dot horseradish peroxidase enzyme-linked staphylococcal protein A immunosorbent assay (Dot-PPA-ELISA) for diagnosis of S. suis infection. The antigen-antibody reaction was optimised through checkerboard titration involving serial dilutions, followed by selective blocking tests and evaluations of cross-reaction, repeatability, and stability. Comparative analysis by using a conventional plate ELISA kit showed that the specificity and sensitivity of the Dot-PPA-ELISA were 97.5 and 96.6%, respectively. Furthermore, dynamic changes in the levels of antibody in rabbits immunised with a propolis inactivated vaccine were monitored by Dot-PPA-ELISA. A total seroprevalence of 73.1% in 305 pig serum samples indicated the method's applicability to detect S. suis infection. Cumulatively, the results suggested that Dot-PAA-ELISA is a convenient, rapid, sensitive, and specific diagnostic method suitable for studying large numbers of samples obtained from clinical and epidemiological studies, thereby helping reduce important economic losses.
- Subjects
GLUTAMATE dehydrogenase; ANTIGEN processing; STREPTOCOCCUS suis; ZOONOSES; HORSERADISH peroxidase; DIAGNOSIS
- Publication
Antonie van Leeuwenhoek, 2017, Vol 110, Issue 4, p585
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-016-0825-z