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- Title
Pusillimonas caeni sp. nov., isolated from a sludge sample of a biofilm reactor.
- Authors
Jin, Long; Ko, So-Ra; Cui, Yingshun; Lee, Chang; Oh, Hee-Mock; Ahn, Chi-Yong; Lee, Hyung-Gwan
- Abstract
A polyphasic taxonomic study was carried out on strain EBR-8-1 isolated from a biofilm reactor in Korea. The cells of the strain were Gram-stain negative, non-spore-forming, non-motile, and short rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this strain with Betaproteobacteria, which showed high pairwise sequence similarities with Pusillimonas noertemannii BN9 (99.1 %), Pusillimonas soli MJ07 (97.3 %), Pusillimonas ginsengisoli DCY25 (97.2 %), and Pusillimonas harenae B201 (96.8 %). The phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage within the genus Pusillimonas. The major fatty acids were identified as C, C cyclo and C cyclo ω8 c. The major cellular polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The respiratory quinone was identified as Q-8 and the genomic DNA G+C content was determined to be 63.3 mol%. On the basis of polyphasic evidence, it is proposed that strain EBR-8-1 should be placed in a new species, Pusillimonas caeni sp. nov. The type stain is EBR-8-1 (=KCTC 42353 = JCM 30463).
- Subjects
SEWAGE sludge microbiology; BIOFILMS; RIBOSOMAL RNA; RNA sequencing; PROTEOBACTERIA
- Publication
Antonie van Leeuwenhoek, 2017, Vol 110, Issue 1, p125
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-016-0782-6