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- Title
Anaerobic methane oxidation coupled to nitrite reduction can be a potential methane sink in coastal environments.
- Authors
Shen, Li-dong; Hu, Bao-lan; Liu, Shuai; Chai, Xiao-ping; He, Zhan-fei; Ren, Hong-xing; Liu, Yan; Geng, Sha; Wang, Wei; Tang, Jing-liang; Wang, Yi-ming; Lou, Li-ping; Xu, Xiang-yang; Zheng, Ping
- Abstract
In the current study, we investigated nitrite-dependent anaerobic methane oxidation (N-DAMO) as a potential methane sink in the Hangzhou Bay and the adjacent Zhoushan sea area. The potential activity of the N-DAMO process was primarily observed in Hangzhou Bay by means of C-labeling experiments, whereas very low or no potential N-DAMO activity could be detected in the Zhoushan sea area. The measured potential N-DAMO rates ranged from 0.2 to 1.3 nmol CO g (dry sediment) day, and the N-DAMO potentially contributed 2.0-9.4 % to the total microbial methane oxidation in the examined sediments. This indicated that the N-DAMO process may be an alternative pathway in the coastal methane cycle. Phylogenetic analyses confirmed the presence of Candidatus Methylomirabilis oxyfera-like bacteria in all the examined sediments, while the group A members (the dominant bacteria responsible for N-DAMO) were found mainly in Hangzhou Bay. Quantitative PCR showed that the 16S rRNA gene abundance of Candidatus M. oxyfera-like bacteria varied from 5.4 × 10 to 5.0 × 10 copies g (dry sediment), with a higher abundance observed in Hangzhou Bay. In addition, the overlying water NO concentration and salinity were identified as the most important factors influencing the abundance and potential activity of Candidatus M. oxyfera-like bacteria in the examined sediments. This study showed the evidence of N-DAMO in coastal environments and indicated the importance of N-DAMO as a potential methane sink in coastal environments.
- Subjects
HANGZHOU Bay (China); METHANE; OXIDATION; NITRITE reductase; SEDIMENTS; CANDIDATUS
- Publication
Applied Microbiology & Biotechnology, 2016, Vol 100, Issue 16, p7171
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-016-7627-0