We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Dexmedetomidine protects against high mobility group box 1‐induced cellular injury by inhibiting pyroptosis.
- Authors
Ji, Xuexia; Guo, Yuanbo; Zhou, Guobin; Wang, Yan; Zhang, Jianxing; Wang, Zhipeng; Wang, Qing
- Abstract
Dexmedetomidine (DEX) is a widely used clinical anesthetic with proven anti‐inflammatory effects. Both high mobility group box 1 (HMGB1) and pyroptosis play an important role in the inflammatory response to infection and trauma. Thus far, there have been no studies published addressing the effect of DEX on HMGB1 and pyroptosis. In order to fill this gap in the literature, bone marrow‐derived macrophages (BMDMs) were exposed to HMGB1 (4 µg/mL) with or without DEX (50 μM) pretreatment. The production of pro‐inflammatory cytokines [such as tumor necrosis factor α (TNF‐α), interleukin 1β (IL‐1β), and IL‐18], phosphorylation of extracellular signal‐regulated protein kinases 1 and 2 (ERK1/2) and P38, and the activation of caspase‐1 were measured by enzyme immunosorbent assay, western blot analysis, confocal microscope, and flow cytometry, respectively. We found that DEX protected against HMGB1‐induced cell death of BMDMs. In addition, DEX suppressed the generation of TNF‐α, IL‐1β, and IL‐18 as well as the phosphorylation of ERK1/2 and P38. Moreover, DEX inhibited caspase‐1 activation and decreased pyroptosis. Taken together, these findings demonstrate the protective effect of DEX in mediating HMGB1‐induced cellular injury, thus indicating that DEX may be a potential therapeutic candidate for the management of infection and trauma‐derived inflammation.
- Subjects
DEXMEDETOMIDINE; CASPASES; HIGH mobility group proteins; MACROPHAGES; CYTOKINES
- Publication
Cell Biology International, 2019, Vol 43, Issue 6, p651
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1002/cbin.11140