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- Title
AMPK and vacuole-associated Atg14p orchestrate μ-lipophagy for energy production and long-term survival under glucose starvation.
- Authors
Seo, Arnold Y.; Pick-Wei Lau; Feliciano, Daniel; Sengupta, Prabuddha; Gros, Mark A. Le; Cinquin, Bertrand; Larabell, Carolyn A.; Lippincott-Schwartz, Jennifer
- Abstract
Dietary restriction increases the longevity of many organisms, but the cell signaling and organellar mechanisms underlying this capability are unclear. We demonstrate that to permit long-term survival in response to sudden glucose depletion, yeast cells activate lipid-droplet (LD) consumption through micro-lipophagy (μ-lipophagy), in which fat is metabolized as an alternative energy source. AMP-activated protein kinase (AMPK) activation triggered this pathway, which required Atg14p. More gradual glucose starvation, amino acid deprivation or rapamycin did not trigger μ-lipophagy and failed to provide the needed substitute energy source for long-term survival. During acute glucose restriction, activated AMPK was stabilized from degradation and interacted with Atg14p. This prompted Atg14p redistribution from ER exit sites onto liquidordered vacuole membrane domains, initiating μ-lipophagy. Our findings that activated AMPK and Atg14p are required to orchestrate μ-lipophagy for energy production in starved cells is relevant for studies on aging and evolutionary survival strategies of different organisms.
- Subjects
RENEWABLE energy sources; GLUCOSE; STARVATION; PROTEIN kinases; SURVIVAL
- Publication
eLife, 2017, p1
- ISSN
2050-084X
- Publication type
Article
- DOI
10.7554/eLife.21690.001