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- Title
Cytofluorometric measurements of nuclear DNA in adventitious buds and shoots of Picea abies regenerated in vitro.
- Authors
Hakman, I.; von Arnold, S.; Bengtsson, A.
- Abstract
Nuclear DNA content of adventitious buds and shoots of Picea abies (L.) Karst. developed in vitro was compared to that of buds collected from field-grown trees. Protoplasts were isolated from the different tissues and after fixation and staining with ethidium bromide the DNA content of their interphase nuclei was determined cytofluorometrically. The DNA pattern of the different tissues was within the same range and had the same distribution with one main peak.
- Subjects
CYTOPHOTOMETRY; GYMNOSPERMS; NORWAY spruce; PROTOPLASTS; TISSUE culture; CYTOLOGICAL techniques
- Publication
Physiologia Plantarum, 1984, Vol 60, Issue 3, p321
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1984.tb06070.x